Background: Thyroid hormones have an important role for the body metabolism. Hyperthyroidism is a condition when thyroid hormone increases due to abnormalities in thyroid gland. Hyperthyroidism is a second largest endocrine disease in Indonesia, some risk factors that aggravate age, gender and smoking. Radioiodine (I-131) is one of the effective therapies for high uptake hyperthyroidism. Objective: The purpose of this study is to determine characteristics and therapeutic effect of radioiodine (I-131) to TSH, T3 and FT4 hormone levels hyperthyroidism high uptake patients in Nuclear Medicine Department, Dr. Hasan Sadikin Hospital Bandung, Indonesia 2014-2015 periods.