Detail Inovasi Perguruan Tinggi


Tema: The 2nd International Seminar and Expo on Jamu
Judul: DIFFERENT EXTRACTION TECHNIQUES AND SOLVENTS INTERFERE PHENOL CONTENTS IN ANTIOXIDANT PROPERTY FROM ROSELLA LEAF EXTRACTS
Perguruan Tinggi: Universitas Jenderal Achmad Yani
Jenis/sdm: dosen/ 0416108405

Tahun: 2017

The Rosella plant (Hibiscus sabdariffa L.) is one of the natural antioxidant source. Each part of the plant (flowers, calyces, stems, roots, seeds, and leaves) has an antioxidant capability. The objective of this research is to find the correlation between extraction techniques, solvents use and total phenol contents with antioxidant properties from four Rosella leaf extracts. The differences among the extracts are in the technique of extraction (maceration, infusion, reflux) and solvents use (water, ethanol). The extracts using maceration and infusion techniques with water solvent are AM, AI. The extracts using maceration and reflux techniques with ethanol solvent are EM, ER. Determination of total phenol content performed by Folin-Ciocalteu reagent. Phenol contents of AM, AI, EM, ER, are 9.62 mg/g, 5.96 mg/g, 17.26 mg/g, 16.92 mg/g of extract as expressed in tannic acid equivalents . The highest phenol contents was identified in ER. By means, the chromatogram pattern in all various extracts using DPPH 0.2% as spray detection for the antioxidant activities are identified. Determination of antioxidant property from four different extracts of Rosella leaf were performed by DPPH (1,1-diphenyl-2-picrylhydrazyl) method. The IC50 value from AM, AI, EM and ER extracts are 0,00056 ppm (very strong), 0,00057 (very strong), 0,00044 ppm (very strong), 0,00092 ppm (very strong), respectively. The result indicated that ethanol extract with maceration technique (EM) possesses more antioxidant activities compared to the others. The phenol contents and antioxidant activity of the four Rosella leaf extracts depended on extraction techniques and solvent uses. Keywords: Rosella, leaf extracts, phenols, Folin-Ciocalteu, DPPH