Suruhan (Peperomia pellucida) is easily found in Indonesia and has the potential of being a herb medicine. General phytochemical screening revealed the presence of flavonoid, steroid, triterpenoid, alkaloid and tannin compounds. Two steroid compounds were isolated from ethyl acetate fraction of the Peperomia pellucida. The compounds were isolated with chromatography method and antimalarial activity test with Desjardin method. Based on the spectral evidence IR, 1 H NMR, 13C NMR, 2D NMR and MS spectroscopies, structures were determined to be stigmasterol (1) and fucosterol (2). The results of antimalarial activity test showed IC50 value of stigmasterol is 5.24 ppm and fucosterol is 0.85 ppm. This is the first report so far of isolation of steroid compounds from Peperomia pellucida plant from Gorontalo, Indonesia and their antimalarial activity test.